Weight LossEnergyMuscleSubcutaneous

AICAR

50mg Vial

Typical Dose

25–50 mg

Frequency

Once daily

Half-Life

~2–3 hours

Research-use dosing; specific study population not established.

AICAR Overview

Mechanism of Action

AICAR (5-Aminoimidazole-4-carboxamide ribonucleoside) is an AMP-activated protein kinase (AMPK) activator. Once phosphorylated to ZMP, it mimics AMP and activates AMPK — the master metabolic switch. AMPK activation increases glucose uptake, fatty acid oxidation, mitochondrial biogenesis, and autophagy — the same adaptations triggered by endurance exercise.

Half-Life

~2–3 hours

Timing

Morning or pre-exercise

Injection Site

Subcutaneous

Food

Fasting may enhance AMPK activation

Common Stacks

  • SLU-PP-332 — Complementary exercise mimetic (ERR)
  • MOTS-c — Synergistic AMPK activation
  • NAD+ — Cellular energy

AICAR Dosing Protocol

Standard Protocol

4–8 weeks

25–50 mg SQ daily

Often cycled 4 weeks on / 2 off.

AICAR Expected Timeline

Week 1–2

Enhanced endurance; fat utilization

Week 3–4

Increased mitochondrial density

Week 4–8

Improved metabolic efficiency

AICAR Side Effects

Hypoglycemia (possible)

Limited human safety data

Injection site irritation

AICAR Research Studies

AMPK activation by AICAR enhances endurance in mice

Narkar VA, et al. — Cell (2008)

Landmark study: AICAR improved running endurance by 44% in sedentary mice without exercise training.

PubMed: 18674809

AICAR FAQ

Can AICAR replace exercise?

Activates AMPK-dependent adaptations but cannot replicate all exercise benefits. Most valuable for those with limited exercise capacity.

Is AICAR banned in sports?

Yes. WADA banned AICAR in 2009 under ‘metabolic modulators’ for its exercise-mimicking effects.

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